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  1. Experimental

qPCR

What primer/probe sequences did you use to quantify the final PCR library with qPCR before submitting to sequencing?

We use Invitrogen Platinum qPCR Supermix w/ROX and these primers:

DLP: [6FAM] CCCTACACGACGCTCTTCCGATCT [TAMRA]

Primer 1: AATGATACGGCGACCACCGAGATC

Primer 2: CAAGCAGAAGACGGCATACGAGATC

PreviousPCR ProductsNextIllumina Sequencing

Last updated 3 years ago

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