> For the complete documentation index, see [llms.txt](https://clipforum.flow.bio/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://clipforum.flow.bio/experimental/qpcr.md).

# qPCR

**What primer/probe sequences did you use to quantify the final PCR library with qPCR before submitting to sequencing?**

We use Invitrogen Platinum qPCR Supermix w/ROX and these primers:

DLP: \[6FAM] CCCTACACGACGCTCTTCCGATCT \[TAMRA]

Primer 1: AATGATACGGCGACCACCGAGATC

Primer 2: CAAGCAGAAGACGGCATACGAGATC<br>