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qPCR
What primer/probe sequences did you use to quantify the final PCR library with qPCR before submitting to sequencing?
We use Invitrogen Platinum qPCR Supermix w/ROX and these primers:
DLP: [6FAM] CCCTACACGACGCTCTTCCGATCT [TAMRA]
Primer 1: AATGATACGGCGACCACCGAGATC
Primer 2: CAAGCAGAAGACGGCATACGAGATC
Last modified 2yr ago