# qPCR **What primer/probe sequences did you use to quantify the final PCR library with qPCR before submitting to sequencing?** We use Invitrogen Platinum qPCR Supermix w/ROX and these primers: DLP: \[6FAM] CCCTACACGACGCTCTTCCGATCT \[TAMRA] Primer 1: AATGATACGGCGACCACCGAGATC Primer 2: CAAGCAGAAGACGGCATACGAGATC