# Demultiplexing **Are the indices in line with read 1? They are also shorter than what our sequencing facility normally uses, are they hard to demultiplex?** We developed a tool for demultiplexing libraries with complex barcoding (including in-line barcodes), as is the case of iCLIP. The tool is called Ultraplex, it's fast and easy to use, please find here: . See Fig1 for an example read from a typical iCLIP library, and the demultiplexing workflow. This tool is also implemented within , a free platform for password-protected data analysis dedicated especially to CLIP. --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://clipforum.flow.bio/computational/demultiplexing.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.