# miCLIP (methylation iCLIP) **Is cDNA truncation the same in the miCLIP protocol for analyzing m6A positions with Abcam and Sysy antibodies to m6A?** Yes, [miCLIP can be used to map the methyl-5-cytosine (m5C) or methyl-6-adenosine (m6A) modifications in RNA by exploiting cDNA truncation](https://www.dropbox.com/s/71dn55vr82gc4sv/2017_miCLIP%20review.pdf?dl=0). In case of antibodies against m6A, a polypeptide is left at the cross-linked nucleotide after proteinase K digestion of the antibody, because the antibody is covalently crosslinked to the RNA in the same way as an RNA-binding protein would be crosslinked in the case of iCLIP. Apart from crosslinking, the rest of the protocol of miCLIP is very similar to iCLIP. --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://clipforum.flow.bio/experimental/miclip-methylation-iclip.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.