LogoLogo
  • Welcome to the CLIP Forum
  • Experimental
    • Cell lysis
    • SDS-Page
    • Crosslinking
    • On-bead biochemistry
    • Adapters and Primers
    • Alternative cDNA library prep protocols
    • cDNA purification
    • Enzymes
    • PCR Products
    • qPCR
    • Illumina Sequencing
    • miCLIP (methylation iCLIP)
    • CLIP-rtPCR (site-specific analysis)
    • iiCLIP protocol
  • Computational
    • Demultiplexing
    • Use of random barcode in data analysis
    • Mapping to repetitive elements/RNAs
    • Differential CLIP binding analysis
  • To be Answered
  • References
Powered by GitBook
On this page

Was this helpful?

  1. Experimental

miCLIP (methylation iCLIP)

PreviousIllumina SequencingNextCLIP-rtPCR (site-specific analysis)

Last updated 3 years ago

Was this helpful?

Is cDNA truncation the same in the miCLIP protocol for analyzing m6A positions with Abcam and Sysy antibodies to m6A?

Yes, . In case of antibodies against m6A, a polypeptide is left at the cross-linked nucleotide after proteinase K digestion of the antibody, because the antibody is covalently crosslinked to the RNA in the same way as an RNA-binding protein would be crosslinked in the case of iCLIP. Apart from crosslinking, the rest of the protocol of miCLIP is very similar to iCLIP.

miCLIP can be used to map the methyl-5-cytosine (m5C) or methyl-6-adenosine (m6A) modifications in RNA by exploiting cDNA truncation