> For the complete documentation index, see [llms.txt](https://clipforum.flow.bio/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://clipforum.flow.bio/experimental/miclip-methylation-iclip.md).

# miCLIP (methylation iCLIP)

**Is cDNA truncation the same in the miCLIP protocol for analyzing m6A positions with Abcam and Sysy antibodies to m6A?**

Yes, [miCLIP can  be used to map the methyl-5-cytosine (m5C) or methyl-6-adenosine (m6A) modifications in RNA by exploiting cDNA truncation](https://www.dropbox.com/s/71dn55vr82gc4sv/2017_miCLIP%20review.pdf?dl=0). In case of antibodies against m6A, a polypeptide is left at the cross-linked nucleotide after proteinase K digestion of the antibody, because the antibody is covalently crosslinked to the RNA in the same way as an RNA-binding protein would be crosslinked in the case of iCLIP. Apart from crosslinking, the rest of the protocol of miCLIP is very similar to iCLIP.