# miCLIP (methylation iCLIP)

**Is cDNA truncation the same in the miCLIP protocol for analyzing m6A positions with Abcam and Sysy antibodies to m6A?**

Yes, [miCLIP can  be used to map the methyl-5-cytosine (m5C) or methyl-6-adenosine (m6A) modifications in RNA by exploiting cDNA truncation](https://www.dropbox.com/s/71dn55vr82gc4sv/2017_miCLIP%20review.pdf?dl=0). In case of antibodies against m6A, a polypeptide is left at the cross-linked nucleotide after proteinase K digestion of the antibody, because the antibody is covalently crosslinked to the RNA in the same way as an RNA-binding protein would be crosslinked in the case of iCLIP. Apart from crosslinking, the rest of the protocol of miCLIP is very similar to iCLIP.