# Enzymes

**Is it necessary to use same companies for enzymes in the protocol, such as Fermentas for BamHI or Invitrogen for polymerase (Accuprime Supermix 1)?**

NEB BamHI would work just as well. Otherwise, we would recommend some testing before changing companies.

**Can the Invitrogen platinum Hot start PCR mix be used over the Invitrogen AccuPrime SuperMix I for the final PCR amplification stage?** 

Certainly. We ourselves have lately switched to Phusion HF Master mix, which is more efficient (it brings down PCR by several cycles, without much change in library quality).<br>