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- How to accurately compare two CLIP libraries of the same RBP with ~10-fold difference of CLIPped RBP abundance in the cell? How can we account for RBP/target RNA ratio?
- Is it necessary to use spike ins, and if so, what kind of spike ins and how should I analyse this?
- How do I choose/remove redundant annotations from genome annotation files?
- How can I compare multiple RBP baits with different IP efficiencies?
- What are the best ways to access and analyze published CLIP data?
- What available pipelines are out there for analysing different CLIP datasets (iCLIP & PAR-CLIP)? Are there advantages/disadvantages to different established pipelines?
- Which statistical methods are best at identifying cross-link sites?
Last modified 2yr ago